SOD1 ELISA Kit (Sheep) (OKCA01964)

Catalog No: OKCA01964

Size:96 Wells

Sheep SOD1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Sheep
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology SOD1 ELISA Kit (Sheep) (OKCA01964) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SOD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SOD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SOD1 captured in the well.
• ELISA Kit Range: 37.5 - 2,400 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	109	95-113
  EDTA Plasma (n=4)	99	91-102

• ELISA Kit Component:
  • SOD1 Microplate: 96 Wells (12 x 8 Well strips)
  • SOD1 Lyophilized Standard: 2 x 2.4 ug
  • 100X Biotinylated SOD1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 9.38 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: SOD1
• Gene Full Name: Superoxide dismutase [Cu-Zn]
• Alias Symbols: ALS, ALS1, IPOA, SOD, homodimer, Cu/Zn superoxide dismutase|SOD, soluble|indophenoloxidase A|superoxide dismutase, cystolic
• Protein Name: Superoxide dismutase [Cu-Zn]
• Description of Target: Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
• Uniprot ID: P09670
• Protein Accession #: XP_004012675.2
• Nucleotide Accession #: XM_004012626.3