SOD (Cu/Zn) Antibody (OASE00355)

Catalog No: OASE00355 (Formerly GWB-3BBBAE)

Size:100ug

Western blot analysis of Rat Tissue lysates showing detection of SOD2 protein using Rabbit Anti-SOD2 Polyclonal Antibody. Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Product Info

• Predicted Species Reactivity: Human, Rat, Mouse, Bovine, Monkey, Invertebrate, Coral, Dog, Hamster, Pig, Rabbit, Sheep, Xenopus, Mollusk, Fish
• Product Format: Liquid. In PBS pH7.0, 50% glycerol, 0.1% sodium azide
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: WB, IHC, IP, ELISA
  Certificate of Analysis: 0.2 ug/mL was sufficient for detection of Cu/Zn SOD in 20ug of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Synthetic Human Cu/Zn SOD Peptide
• Purification: Protein A Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~23kDa (human) and ~19kDa (other species).
• Dilution: WB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.
• Reference: 1.Adachi, T, et al. (1992). Clin. Chim. Acta. 212: 89-102. 2.Barrister, J.V.,et al. (1987). Crit. Rev. Biochem. 22:111-180.
  3.Furukawa,Y; O'Halloran, T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6.
  4.Gao, B, et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925.
  5.Hassan, H.M. (1988). Free Radical Biol. Med. 5: 377-385.
  6.Kurobe, N, et al. (1990) Biomedical Research. 11: 187-194
  7.Ojika, T. et al. (1991). Acta Histochem Cytochem. 24(5): 489-495.
  8.Shinder, G.A. et al. (2001). J. Biol. Chem. 276(16):12791-12796.
  9.Wispe, JR et al. (1989) BBA. 994: 30-36.
• Storage Buffer: PBS pH7.0, 50% glycerol, 0.09% sodium azide
• Publications:

  PYP1-4 peptide from Pyropia yezoensis protects against acetaminophen-induced hepatotoxicity in HepG2 cells. Exp Ther Med. 19, 849-860 (2020). 32010245

• Description:
  View product citations for SOD (Cu/Zn) Antibody (OASE00355) on CiteAb

Target Info

• Gene Symbol: SOD1
• Gene Full Name: superoxide dismutase 1, soluble
• Alias Symbols: Superoxide dismutase1, ALS1, IPOA, SOD1, SOD2, SODC
• NCBI Gene Id: 6647
• Description of Target: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4).
  There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(9). SOD3 can also be distinguished by its heparin-binding capacity (1).
• Uniprot ID: P00441
• Protein Accession #: NP_000445.1