SNAP25 C.elegans Protein (OPPA01308)

Catalog No: OPPA01308 (Formerly GWB-52EA16)

Size:10UG

Product Info

• Product Format: The protein contains 20mM Tris-HCl pH7.5, 50mM NaCl, 5mMDTT, 1mM EDTA and 10% Glycerol. Physical appearance: Sterile filtered colorless solution.
• Host: E. Coli
  Product Introduction: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters.
  Product Description: Recombinant C.elegans SNAP-25 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23kDa. SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.
• Reconstitution and Storage: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Avoid multiple freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERCEGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQPRITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK.

Target Info

• Gene Symbol: SNAP-25
• Alias Symbols: SNAP-25B, SNAP-25a
• NCBI Gene Id: 25012
• Protein Name: Synaptosomal-associated protein 25
• Description of Target: Recombinant Synaptosomal-associated protein 25kDa, C.elegans
• Uniprot ID: P60881
• Protein Accession #: NP_112253.1
• Protein Size (# AA): Recombinant