Catalog No: OKCD08645
Size:96WELLS
Price: $800.00
SKU
OKCD08645
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for SLC7A10 ELISA Kit (Human) (OKCD08645) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asc Type Amino Acid Transporter 1 (ASC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Asc Type Amino Acid Transporter 1 (ASC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Asc Type Amino Acid Transporter 1 (ASC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Asc Type Amino Acid Transporter 1 (ASC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asc Type Amino Acid Transporter 1 (ASC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asc Type Amino Acid Transporter 1 (ASC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.055ng/mL |
Gene Symbol | SLC7A10 |
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Gene Full Name | solute carrier family 7 member 10 |
Alias Symbols | asc-1;ASC1;asc-type amino acid transporter 1;HASC-1;solute carrier family 7 (neutral amino acid transporter light chain, asc system), member 10;Solute carrier family 7 member 10;solute carrier family 7, (cationic amino acid transporter, y+ system) member 10;solute carrier family 7, (neutral amino acid transporter, y+ system) member 10. |
NCBI Gene Id | 56301 |
Protein Name | Asc-type amino acid transporter 1 |
Description of Target | Sodium-independent, high affinity transport of small neutral D- and L-amino acids. May play a role in the modulation of glutamatergic transmission through mobilization of D-serine at the glutamatergic synapse. |
Uniprot ID | Q9NS82 |
Protein Accession # | NP_062823.1 |
Nucleotide Accession # | NM_019849.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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