SFTPA1 ELISA Kit (Mouse) (OKCD02903)

Catalog No: OKCD02903

Size:96WELLS

Mouse SFTPA1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	89-96%	81-96%	88-97%
  EDTA Plasma (n=5)	87-103%	98-105%	95-105%	95-103%
  Heparin Plasma (n=5)	87-101%	79-94%	96-103%	91-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pulmonary Surfactant Associated Protein A1 (SFTPA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pulmonary Surfactant Associated Protein A1 (SFTPA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pulmonary Surfactant Associated Protein A1 (SFTPA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pulmonary Surfactant Associated Protein A1 (SFTPA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-88	83
  EDTA Plasma (n=5)	80-88	84
  Heparin Plasma(n=5)	84-92	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pulmonary Surfactant Associated Protein A1 (SFTPA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pulmonary Surfactant Associated Protein A1 (SFTPA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SFTPA1 Microplate: 96 Wells (12 x 8 Well strips)
  • SFTPA1 Lyophilized Standard: 2
  • 100X Biotinylated SFTPA1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, lung lavage fluid and other biological fluids
• Sensitivity: 11.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Surfactant Associated Protein A (SPA). No significant cross-reactivity or interference between Surfactant Associated Protein A (SPA) and analogues was observed
• Publications:

  Change of surfactant protein D and A after renal ischemia reperfusion injury. PLoS One. 14, e0227097 (2019). 31877195

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for SFTPA1 ELISA kit (Mouse) on CiteAb

Target Info

• Gene Symbol: SFTPA1
• Alias Symbols: PSAP, PSP-A, Pulmonary surfactant-associated protein A, Sftp1, Sftp-1, Sftpa, SFTPA1, SP-A, surfactant pulmonary associated protein A1
• Protein Name: Pulmonary surfactant-associated protein A
• Description of Target: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages (PubMed:25965346).
• Uniprot ID: P35242
• Protein Accession #: NP_075623.2
• Nucleotide Accession #: NM_023134.4
• Protein Size (# AA): 248