SERPINB4 ELISA Kit (Human) (OKCD00223)

Catalog No: OKCD00223 (Formerly GWB-KBBBA8)

Size:96WELLS

Human SERPINB4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-101%	86-99%	99-105%	92-99%
  EDTA plasma(n=5)	93-101%	87-98%	79-96%	95-104%
  heparin plasma(n=5)	96-105%	85-93%	78-98%	80-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serpin Peptidase Inhibitor,Clade B,Member 4 (SERPINB4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serpin Peptidase Inhibitor,Clade B,Member 4 (SERPINB4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serpin Peptidase Inhibitor,Clade B,Member 4 (SERPINB4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serpin Peptidase Inhibitor,Clade B,Member 4 (SERPINB4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	78-95	86
  EDTA plasma(n=5)	97-105	102
  heparin plasma(n=5)	83-90	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serpin Peptidase Inhibitor,Clade B,Member 4 (SERPINB4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serpin Peptidase Inhibitor,Clade B,Member 4 (SERPINB4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SERPINB4 Microplate: 96 Wells (12 x 8 Well Strips)
  • SERPINB4 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SERPINB4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Squamous Cell Carcinoma Antigen 2 (SCCA2). No significant cross-reactivity or interference between Squamous Cell Carcinoma Antigen 2 (SCCA2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SERPINB4
• Gene Full Name: serpin family B member 4
• Alias Symbols: PI11, SCCA1, SCCA2, LEUPIN, SCCA-2
• NCBI Gene Id: 6318
• Protein Name: Squamous cell carcinoma antigen 2
• Description of Target: Squamous cell carcinoma antigen 2
• Uniprot ID: Q86W05
• Protein Accession #: NP_002965
• Nucleotide Accession #: NM_002974.3
• Protein Size (# AA): 390