SCUBE1 ELISA Kit (Rat) (OKEI00879)

Catalog No: OKEI00879

Size:96 Wells

Rat SCUBE1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Scube1 ELISA Kit (Rat) (OKEI00879) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Scube1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Scube1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Scube1
• ELISA Kit Range: 0.625 - 40 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Scube1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Scube1 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • LACTB2 Microplate: 96 Wells (12 x 8 Well strips)
  • LACTB2 Lyophilized Standard: 2
  • 100X LACTB2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.38 ng/mL
• Publications:

  SCUBE1 Controls BMPR2-Relevant Pulmonary Endothelial Function: Implications for Diagnostic Marker Development in Pulmonary Arterial Hypertension. JACC Basic Transl Sci. 5, 1073-1092 (2020). 33294740

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for SCUBE1 ELISA Kit (Rat) (OKEI00879) on CiteAb

Target Info

• Gene Symbol: SCUBE1
• Alias Symbols: Signal peptide, CUB domain and EGF-like domain-containing 1
• NCBI Gene Id: 315174
• Protein Name: Signal peptide, CUB domain and EGF-like domain-containing 1
• Uniprot ID: F1M987
• Protein Accession #: NP_001128356
• Nucleotide Accession #: NM_001134884