SCAF11 ELISA Kit (Human) (OKCD05917)

Catalog No: OKCD05917

Size:96WELLS

SCAF11 ELISA Kit (Human) (OKCD05917) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein SCAF11 (SCAF11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein SCAF11 (SCAF11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein SCAF11 (SCAF11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein SCAF11 (SCAF11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein SCAF11 (SCAF11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein SCAF11 (SCAF11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SCAF11 Microplate: 96 Wells (12 x 8 Well strips)
  • SCAF11 Lyophilized Standard: 2
  • 100X SCAF11 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.059ng/mL

Target Info

• Gene Symbol: SCAF11
• Gene Full Name: SR-related CTD associated factor 11
• Alias Symbols: CASP11;CTD-associated SR protein 11;protein SCAF11;renal carcinoma antigen NY-REN-40;SC35-interacting protein 1;serine/arginine-rich splicing factor 2-interacting protein;SFRS2-interacting protein;SFRS2IP;SIP1;splicing factor, arginine/serine-rich 2-interacting protein;splicing regulatory protein 129;SR-related and CTD-associated factor 11;SRRP129;SRSF2-interacting protein;SRSF2IP.
• NCBI Gene Id: 9169
• Protein Name: Protein SCAF11
• Description of Target: Plays a role in pre-mRNA alternative splicing by regulating spliceosome assembly.
• Uniprot ID: Q99590
• Protein Accession #: NP_004710.2
• Nucleotide Accession #: NM_004719.2