SARS-CoV-2 Spike Antibody (OAPB00314)

Catalog No: OAPB00314

Size:100UG

Click here to learn more about Aviva's By-Request Conjugation Service.

Figure 1 ELISA Test
Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody (1 ug/mL). A sandwich ELISA was performed using antigen or control peptide as coating antigen and the anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 ng/mL to 1000ng/mL.

Product Info

• Predicted Species Reactivity: SARS-CoV-2 / COVID-19 Virus|Severe Acute Respiratory Syndrome Coronavirus|Virus
• Product Format: Liquid. SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike antibody (3525) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (100%)
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-S (OAPB00314) antibody is Catalog # OEPB02407
• Application Info: WB: 1 ug/mL
  IF: 1 ug/mL
  IHC: 0.2 ug/mL
  IF and WB in human samples. IHC and IF in COVID-19 patient samples. SARS-CoV-2 (COVID-19) Spike antibody can be used for the detection of SARS-CoV-2 Spike protein in ELISA. It will detect 4 ng of free peptide at 1 ug/mL. All other applications and species not yet tested.
• Reference: 1) Gorbalenya. bioRxiv: 2020.
  2) Hui et al. Int J Infect Dis. 2020;91:264-266.
  3) Belouzard et al. Viruses. 2012;4(6):1011-33.
  4) Lee et al. J Virol. 2006;80(8):4079-87.
  5) Wan et al. J Virol. 2020.
  6) Wrapp et al. Science. 2020.

Target Info

• Gene Symbol: S
• Gene Full Name: surface glycoprotein
• Alias Symbols: E2;GU280_gp02;Peplomer protein;spike glycoprotein;surface glycoprotein.
• NCBI Gene Id: 43740568
• Protein Name: Spike glycoprotein|surface glycoprotein [Severe acute respiratory syndrome coronavirus 2]
• Description of Target: Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32221306, PubMed:32075877, PubMed:32155444). Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell (PubMed:33082294, PubMed:33082293). This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2 (PubMed:33082293). The stalk domain of S contains three hinges, giving the head unexpected orientational freedom (PubMed:32817270). Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Can be alternatively processed by host furin (PubMed:32362314). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes.
• Uniprot ID: P0DTC2
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/QHD43416