S100A1 ELISA Kit (Bovine) (OKEH08074)

Catalog No: OKEH08074

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology S100A1 ELISA Kit (Bovine) (OKEH08074) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100A1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.4% (n = 20)
  Mean Inter-assay CV%: <=4.6% (n = 20)
• ELISA Kit Component:
  • S100A1 Microplate: 96 Wells (12 x 8 Well strips)
  • S100A1 Lyophilized Standard: 2
  • 100X Biotinylated S100A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
  Unigene: Bt.35032
  SMR: P02639
  String: 9913.ENSBTAP00000044226
  KEGG: bta:528735
  Pfam: PF00036
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Protein S100-A1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: S100A1
• Gene Full Name: S100 calcium binding protein A1
• Alias Symbols: protein S100-A1;S100 calcium-binding protein A1;S-100 protein alpha chain;S-100 protein subunit alpha.
• NCBI Gene Id: 528735
• Protein Name: P02639
• Description of Target: Protein S100-A1
• Uniprot ID: https://www.uniprot.org/uniprot/P02639
• Protein Accession #: NP_001092512.1
• Nucleotide Accession #: NM_001099042.2