RuBisCO ELISA Kit (Plant) (OKCA00374)

Catalog No: OKCA00374

Size:96 Wells

Plant RuBisCO ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Plant
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: < 4 Hours
• ELISA Kit Principle: Aviva Systems Biology RuBisCO ELISA Kit (Plant) (OKCA00374) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO and Horseradish Peroxidase (HRP) conjugated RuBisCO. The competitive inhibition reaction is launched between with HRP labeled RuBisCO and unlabeled RuBisCO with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured.
• ELISA Kit Range: 3.13 ug/mL - 800 ug/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  leaf tissue (n=5)	0.95	89-103%

• ELISA Kit Reproducibility:

  Mean Intra-Assay Precision %CV: < 10% (n = 20)

  Mean Inter-Assay Precision %CV: < 20% (n = 20)

• ELISA Kit Component:
  • Anti-RuBisCO Microplate: 96 Well (12 x 8 Well Strips)
  • RuBisCO Standard: 2 x 500 uL
  • 100X Antibody: 1 x 60 uL
  • 100X Anti-RuBisCO Antibody HRP Conjugate: 1 x 120 uL
  • Antibody Diluent: 1 x 10 mL
  • 25X HRP-Conjugate Diluent: 1 x 20 mL
  • Sample Extraction Buffer: 1 x 20 mL
  • Sample Diluent: 2 x 20 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information:

  CAS#: 9027-23-0

• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Plant tissues
• Sensitivity: < 3.13 ug/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of plant
  RuBisCO. No significant cross-reactivity or interference between plant RuBisCO
  and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Immunoassay

Target Info

• Gene Symbol: RuBisCO
• Protein Name: RuBisCO