RNASET2A Chemi-Luminescent ELISA Kit (Mouse) (OKCD03906)

Catalog No: OKCD03906

Size:96WELLS

Mouse RNASET2A ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Ribonuclease T2 (RNASET2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ribonuclease T2 (RNASET2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ribonuclease T2 (RNASET2) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonuclease T2 (RNASET2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonuclease T2 (RNASET2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Rnaset2a Microplate: 96 Wells (12 x 8 Well strips)
  • Rnaset2a Lyophilized Standard: 2
  • 100X Rnaset2a Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.312 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Rnaset2a|Rnaset2b
• Gene Full Name: ribonuclease T2A|ribonuclease T2B
• Alias Symbols: 0610007O07Rik;4833423A10Rik;4930532K22Rik;C79750;ribonuclease 6;ribonuclease 6-A;ribonuclease 6-B;ribonuclease T2;ribonuclease T2-A;ribonuclease T2-B;RN;Rna;RNASE6PL;Rnaset2.
• NCBI Gene Id: 100037283|68195
• Protein Name: Ribonuclease T2-A
• Description of Target: Has ribonuclease activity, with higher activity at acidic pH. May play a role in cellular RNA catabolism. Probably is involved in lysosomal degradation of ribosomal RNA.
• Uniprot ID: C0HKG5
• Protein Accession #: NP_001077407
• Nucleotide Accession #: NM_001083938