RIOK1 ELISA Kit (Human) (OKCD02849)

Catalog No: OKCD02849

Size:96WELLS

Human RIOK1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	97-105%	97-105%	79-90%	83-93%
  EDTA Plasma (n=5)	95-103%	91-98%	78-94%	78-101%
  Heparin Plasma (n=5)	86-93%	91-99%	87-104%	83-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RIO Kinase 1 (RIOK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RIO Kinase 1 (RIOK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RIO Kinase 1 (RIOK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RIO Kinase 1 (RIOK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-98	89
  EDTA Plasma (n=5)	80-94	84
  Heparin Plasma(n=5)	80-103	83

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RIO Kinase 1 (RIOK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RIO Kinase 1 (RIOK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-RIOK1 Microplate: 96 Wells (12 x 8 Well strips)
  • RIOK1 Lyophilized Standard: 2
  • 100X Biotinylated RIOK1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.133 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of RIO Kinase 1 (RIOK1). No significant cross-reactivity or interference between RIO Kinase 1 (RIOK1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RIOK1
• Gene Full Name: RIO kinase 1
• Alias Symbols: AD034;bA288G3.1;RIO kinase 1;RIO1;RRP10;serine/threonine-protein kinase RIO1.
• NCBI Gene Id: 83732
• Protein Name: Serine/threonine-protein kinase RIO1
• Description of Target: Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed:22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (PubMed:21081503).
• Uniprot ID: Q9BRS2
• Protein Accession #: NP_113668.2
• Nucleotide Accession #: NM_031480.2
• Protein Size (# AA): 568