Recombinant SARS-CoV-2 Spike S1 Protein (OPAN06665)

Catalog No: OPAN06665

Size:100UG

Immobilized Recombinant 2019-nCoV Spike S1-His at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 1.5-15 ng/mL.

Recombinant 2019-nCoV Spike S1 protein on Tris-Bis SDS-PAGE under reduced condition. The purity is greater than 95%.

Product Info

• Tested Species Reactivity: 2019-nCoV
• Predicted Species Reactivity: SARS-CoV-2 / COVID-19 Virus, Severe acute respiratory syndrome coronavirus 2
• Product Format: Lyophilized PBS (pH 7.4)
• Application: SDS-PAGE
  Endotoxin Level: < 0.1 EU/ug of the protein by LAL method.
• Reconstitution and Storage: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Avoid repeated freeze/thaw cycles.
• Purity: >95% by SDS-PAGE, HPLC
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1 at 2 ug/mL (100 uL/well) can bind recombinant Human ACE2 with a linear range of 0.15-6.85 ng/mL. Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 24.5 nM as determined in a SPR assay.
• Protein Sequence: Gln14-Arg683 of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6 x His tag and Avi at the C-terminus.
• Source: HEK293 Cells
• Tag:  6 x His tag and Avi at the C-terminus

Target Info

• Gene Symbol: S
• Gene Full Name: surface glycoprotein
• Alias Symbols: GU280_gp02;spike glycoprotein;surface glycoprotein.
• NCBI Gene Id: 43740568
• Protein Name: surface glycoprotein
• Description of Target: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Protein Accession #: YP_009724390.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 78 kDa