Catalog No: OPAN06665
Size:100 ug
Price: $349.00
SKU
OPAN06665
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for OPAN06665
Product Info
Tested Species Reactivity2019-nCoV
Predicted Species Reactivity2019-nCoV
Product FormatLyophilized PBS (pH 7.4)
ApplicationSDS-PAGE
::Endotoxin Level: < 0.1 EU/ug of the protein by LAL method.
Reconstitution and StorageReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Avoid repeated freeze/thaw cycles.
Purity>95% by SDS-PAGE, HPLC
Biological ActivityMeasured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1 at 2 ug/mL (100 uL/well) can bind recombinant Human ACE2 with a linear range of 0.15-6.85 ng/mL. Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 24.5 nM as determined in a SPR assay.
Protein SequenceGln14-Arg683 of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6 x His tag and Avi at the C-terminus.
SourceHEK293 cells
Tag 6 x His tag and Avi at the C-terminus
Gene SymbolS
Alias Symbolsspike glycoprotein
NCBI Gene Id43740568
Protein Namesurface glycoprotein
Description of TargetThe spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Protein Accession #YP_009724390.1
Molecular Weight78 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express S.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express S.
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