RCBTB2 ELISA Kit (Human) (OKCD01078)

Catalog No: OKCD01078 (Formerly GWB-KBBZH1)

Size:96WELLS

Human RCBTB2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RCC1 And BTB Domain Containing Protein 2 (RCBTB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RCC1 And BTB Domain Containing Protein 2 (RCBTB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RCC1 And BTB Domain Containing Protein 2 (RCBTB2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RCC1 And BTB Domain Containing Protein 2 (RCBTB2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-RCBTB2 Microplate: 96 Wells (12 x 8 Well Strips)
  • RCBTB2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated RCBTB2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of RCC1 And BTB Domain Containing Protein 2 (RCBTB2). No significant cross-reactivity or interference between RCC1 And BTB Domain Containing Protein 2 (RCBTB2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: RCBTB2
• Gene Full Name: RCC1 and BTB domain containing protein 2
• Alias Symbols: CHC1L;Chromosome condensation 1-like;RCC1 and BTB domain-containing protein 2;RCC1-like G exchanging factor;RCC1-like G exchanging factor RLG;regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2;Regulator of chromosome condensation and BTB domain-containing protein 2;RLG.
• NCBI Gene Id: 1102
• Protein Name: RCC1 and BTB domain-containing protein 2
• Description of Target: RCC1 and BTB domain-containing protein 2
• Uniprot ID: O95199
• Protein Accession #: NP_001259
• Nucleotide Accession #: NM_001268.3
• Protein Size (# AA): 551