Catalog No: OOTA00710
Price: $0.00
SKU
OOTA00710
Availability: Discontinued
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Tested Species Reactivity | Rat |
---|---|
Application | ELISA |
:: | Capture Antibody: 26 ug of Rabbit Anti-Rat TNF-alpha + 0.5 mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 260 ul sterile water for a concentration of 100 ug/ml. |
:: | Detection Antibody: 51 ug of Biotinylated Rabbit Anti-Rat TNF-alpha + 0.5 mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 510 ul sterile water for a concentration of 100 ug/ml. |
:: | Recombinant Standard: 1 ug of Recombinant-Rat TNF-alpha + 2.2 mg BSA + 11.0 mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 1 ml sterile water for a concentration of 1ug/ml. Note |
:: | Strepavidin-HRP: 17 ul vial. Centrifuge vial prior to opening. Upon receipt, streptavidin-HRP conjugate should be diluted using 153 ul of 1xPBS for a total of 170 ul at a concentration of 100 ug/ml. This solution can then be aliquoted into ten 16 ul vials and stored at 2-8C for at least six months. |
Reconstitution and Storage | Mixed Storage Conditions |
Specificity | TNF-alpha |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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