Mouse CD4/L3T4 Antibody : APC/CY7 (OASB00270)

Catalog No: OASB00270

Size:100UG

Amount Used: 0.2 Ug/106 cells from BALB/c mesenteric lymph nodes were double-stained with anti-mouse CD4-PE and anti-mouse CD8a-FITC, gated on small lymphocytes and analyzed by flow cytometry.

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD4-APC/CY7 and Rat Anti-Mouse CD8a-APC.

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD4-APC/CY7.

Product Info

• Tested Species Reactivity: Mouse
• Predicted Species Reactivity: Mouse
• Clonality: Monoclonal
• Clone: GK1.5
• Isotype: IgG2b
• Host: Rat
• Conjugation: APC-Cyanine 7
• Application: FC
• Additional Information: Description: CD4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. In vivo and in vitro treatment with the GK1.5 monoclonal antibody effectively depletes CD4+ cells. GK1.5 also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Mouse CTL clone V4
• Concentration: 0.1 mg/mL
• Specificity: CD4
• Characterization: To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of astandard reference reagent. Representative data are included in this package insert.
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or faceprotection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physicianimmediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Fluorescein and Biotin conjugates <= 1 ug/10⁶ cells
  R-phycoerythrin conjugate
  Allophycocyanin conjugate
  R-phycoerythrin conjugate-Texas Red®
  Spectral Red conjugate
  Cyanine 5 conjugate
  R-phycoerythrin-Cyanine 5.5 conjugate
  R-phycoerythrin-Cyanine 7 conjugate
  APC-CY5.5 and APC-CY7 conjugates
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
• Application Info: Flow cytometry 1, Immunohistochemistry (FS) 15, Immunoprecipitation 1,2, In vivo and in vitro depletion of CD4+ cells 6,7, Fractionation of CD4+ cells14
• Other Applications Data: Since applications vary, you should determine the optimum working dilution forthe product that is appropriate for your specific need.
• Other Applications Image 1 Data: Amount Used: 0.2 Ug/106 cells Cells from BALB/c mesenteric lymph nodes were double-stained with anti-mouse CD4-PE and anti-mouse CD8a-FITC , gated on small lymphocytes and analyzed by flow cytometry.
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The allophycocyanin (APC) conjugate is supplied as as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 7 (RPE- CY 7), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5 Allophycocyanin- Cyanine 5.5 (APC- CY 5.5),and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
  - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
• Reference: 1. Dialynas, D. P., D. B. Wilde, P. Marrack, et al. 1983. Immunol. Rev. 74:29
  2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933
  3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473
  4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller- Sieburg. 1992. Blood 60:1717
  5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71
  6. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1
  7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1
  8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445
  9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118
  10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808
  11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5
  12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131
  13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207
  14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1
  15. B. Zheng, B., S. Han, and G. Kelsoe. 1996. J. Exp. Med 184:1083- 1091.

Target Info

• Gene Symbol: CD4
• Gene Full Name: T-cell surface glycoprotein CD4
• Alias Symbols: L3T, Ly-, L3T4, Ly-4
• NCBI Gene Id: 12504
• Protein Name: T-cell surface glycoprotein CD4
• Uniprot ID: P06332
• Protein Accession #: NP_038516.1
• Nucleotide Accession #: NM_013488.2