RASGRP4 Antibody (OAGA00338)

Catalog No: OAGA00338

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
OAGA00338 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using RASGRP4 (OAGA00338) antibody at 1:100 dilution.

Immunofluorescence analysis of methanol-fixed HeLa, using RASGRP4(OAGA00338) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  Positive Controls: Jurkat
  Cellular Localization: Cytoplasm, Cell membrane
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 108 and 200 of RASGRP4 (Uniprot ID#Q8TDF6)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: RASGRP4
• Gene Full Name: RAS guanyl-releasing protein 4
• Alias Symbols: RASGRP4 antibody, RAS guanyl-releasing protein 4 antibody, guanyl nucleotide releasing protein 4 antibody, RAS guanyl releasing protein 4 antibody
• NCBI Gene Id: 115727
• Protein Name: RAS guanyl-releasing protein 4
• Uniprot ID: Q8TDF6
• Protein Accession #: NP_001139674.1
• Molecular Weight: 75 kDa