RAD18 Antibody (OAGA01055)

Catalog No: OAGA01055

Size:100 ul

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using RAD18(OAGA01055) antibody at 1:100 dilution.

RAD18 antibody [N1C1] detects RAD18 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAD18 protein stained by RAD18 antibody [N1C1] (OAGA01055) diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Bovine, Pig
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE
  Cellular Localization: Nucleus
  
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RAD18. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Human (100%), Bovine (80%), Pig (84%)
• Concentration: 0.46 mg/ml
• Intended Use: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
• Application Info: ICC/IF: 1:100~1000
  IHC-FFPE: 1:100~1000
  Optimal dilutions/concentrations should be determined by the researcher.

Target Info

• Gene Symbol: RAD18
• Gene Full Name: E3 ubiquitin-protein ligase RAD18
• Alias Symbols: RNF73
• NCBI Gene Id: 56852
• Protein Name: E3 ubiquitin-protein ligase RAD18
• Description of Target: The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
• Uniprot ID: Q9NS91
• Protein Accession #: NP_064550.3
• Molecular Weight: 56 kDa