RAB11A Antibody (OAAB21685)

Catalog No: OAAB21685

Size:200ul

All lanes: Anti-RAB11A Antibody (OAAB21685) at 1:4,000 dilution Lane 1:Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: C2C12 whole cell lysate Lane 4: PC-12 whole cell lysate Lane 5: rat brain lysate Lane 6: 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size: 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

OAAB21685 staining RAB11A in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: 1565CT164.36.80
• Isotype: IgG1, kappa
• Host: Mouse
• Application: WB
• Additional Information: Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RAB11A antibody is generated from a mouse immunized with a purified recombinant protein of human RAB11A.
• Purification: Protein G column purification, followed by dialysis against PBS
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Concentration: 500 ug/mL
• Preservative: 0.09% Sodium Azide
• Formulation: Purified monoclonal antibody, supplied in PBS with 0.09% sodium azide.
• Application Info: IHC-P: 1:25
  WB: 1:4000
• Reference: Drivas G.T.,et al.Oncogene 6:3-9(1991).
  Zahraoui A.,et al.Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases.
  Gromov P.S.,et al.FEBS Lett. 429:359-364(1998).
  Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
  Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
  

Target Info

• Gene Symbol: RAB11A
• Gene Full Name: RAB11A, member RAS oncogene family
• Alias Symbols: YL8
• NCBI Gene Id: 8766
• Description of Target: The protein encoded by this gene belongs to the Rab family of the small GTPase superfamily. It is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P62491
• Protein Accession #: NP_001193765.1
• Nucleotide Accession #: NM_001206836.1
• Molecular Weight: 24 kDa