QPCT ELISA Kit (Human) (OKCD09040)

Catalog No: OKCD09040

Size:96WELLS

QPCT ELISA Kit (Human) (OKCD09040) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutaminyl Peptide Cyclotransferase (QPCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutaminyl Peptide Cyclotransferase (QPCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutaminyl Peptide Cyclotransferase (QPCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutaminyl Peptide Cyclotransferase (QPCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutaminyl Peptide Cyclotransferase (QPCT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutaminyl Peptide Cyclotransferase (QPCT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • QPCT Microplate: 96 Wells (12 x 8 Well strips)
  • QPCT Lyophilized Standard: 2
  • 100X QPCT HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.065ng/mL

Target Info

• Gene Symbol: QPCT
• Gene Full Name: glutaminyl-peptide cyclotransferase
• Alias Symbols: EC;GCT;glutaminyl cyclase;glutaminyl-peptide cyclotransferase;glutaminyl-tRNA cyclotransferase;glutamyl cyclase;QC;sQC.
• NCBI Gene Id: 25797
• Protein Name: Glutaminyl-peptide cyclotransferase
• Description of Target: Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-amyloid-beta. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides.
• Uniprot ID: Q16769
• Protein Accession #: NP_036545.1
• Nucleotide Accession #: NM_012413.3