PSD95 Antibody : HRP (OASE00033-HRP)

Catalog No: OASE00033-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Mouse, Rat, Bovine
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: 6G6
• Isotype: IgG2a
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, ICC, IF
• Additional Information: Background Info: Detects a ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.
  Scientific Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4).
  PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).
  
  Certificate of Analysis: 1ug/ml was sufficient for detection of PSD-95 on 20ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse (No Suggestions) as the secondary.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Recombinant rat PSD-95
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1:250 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22(15): 6415-25.
  2. Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168(1): 117-26.
  3. Kennedy M. (1997) Trends in Neurosci. 6: 264-268.
  4. Irie M. et al. (1997) Science 277(5331): 1511-5.
  5. Cai C. et al. (2006) J Biol Chem. 281: 4267-73.
  6. Yao W.D. et al. (2004) Neuron 41: 625-38.
  7. Cline H. (2005) Curr Biol. 15: R203-5.

Target Info

• Gene Symbol: Dlg4
• Gene Full Name: discs, large homolog 4 (Drosophila)
• Alias Symbols: Dlgh4, PSD95, Sap90
• NCBI Gene Id: 29495
• Uniprot ID: P31016
• Protein Accession #: NP_062567.1