Proteolipid Protein 1, Myelin Antibody ELISA Kit (Human) (OKCD01284)

Catalog No: OKCD01284

Size:96WELLS

Human Proteolipid Protein 1, Myelin ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h, 30min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-91%	84-101%	84-92%	96-105%
  EDTA Plasma (n=5)	87-95%	90-98%	93-105%	78-97%
  Heparin Plasma (n=5)	88-103%	84-101%	90-99%	79-95%

• ELISA Kit Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Proteolipid Protein 1, Myelin Antibody (Anti-PLP1) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Proteolipid Protein 1, Myelin Antibody (Anti-PLP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-91	85
  EDTA Plasma (n=5)	90-99	94
  Heparin Plasma (n=5)	78-91	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Proteolipid Protein 1, Myelin Antibody (Anti-PLP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Proteolipid Protein 1, Myelin Antibody (Anti-PLP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Proteolipid Protein 1, Myelin antibody Microplate: 96 Wells (12 x 8 Well strips)
  • Proteolipid Protein 1, Myelin antibody Lyophilized Standard: 2 x XXX
  • 100X Detector Antibody: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 1.12 ng/mL
• Assay Info: Assay Method: Quantitative Reverse Capture Sandwich ELISA

Target Info

• Gene Symbol: PLP1
• Gene Full Name: proteolipid protein 1
• Alias Symbols: GPM6C;HLD1;lipophilin;major myelin proteolipid protein;MMPL;myelin proteolipid protein;PLP;PLP/DM20;PMD;SPG2.
• NCBI Gene Id: 5354
• Protein Name: Myelin proteolipid protein
• Description of Target: This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
• Uniprot ID: P60201
• Nucleotide Accession #: NM_000533.4