PPIB ELISA Kit (Rat) (OKCD01236)

Catalog No: OKCD01236 (Formerly GWB-KBBIS1)

Size:96WELLS

Rat Ppib ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	94-102%	78-94%	88-95%	85-95%
  EDTA plasma(n=5)	86-95%	89-98%	95-102%	78-93%
  heparin plasma(n=5)	95-104%	84-97%	78-99%	95-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclophilin B (CYPB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclophilin B (CYPB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclophilin B (CYPB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cyclophilin B (CYPB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	101
  EDTA plasma(n=5)	81-97	91
  heparin plasma(n=5)	91-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclophilin B (CYPB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclophilin B (CYPB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ppib Microplate: 96 Wells (12 x 8 Well Strips)
  • Ppib Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated Ppib Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.33 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclophilin B (CYPB). No significant cross-reactivity or interference between Cyclophilin B (CYPB) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Ppib
• Gene Full Name: peptidylprolyl isomerase B
• Alias Symbols: cyclophilin B;CypB;CYP-S1;peptidyl-prolyl cis-trans isomerase B;PPIase B;rotamase B;S-cyclophilin;S-cyclophylin;Scylp.
• NCBI Gene Id: 64367
• Protein Name: Peptidyl-prolyl cis-trans isomerase B
• Description of Target: PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
• Uniprot ID: P24368
• Protein Accession #: NP_071981
• Nucleotide Accession #: NM_022536.2
• Protein Size (# AA): 216