POLQ Antibody (OAAF02053)

Catalog No: OAAF02053

Size:100 ug

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DNA Polymerase thet Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human POLQ.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: DNA Polymerase theta antibody detects endogenous levels of total DNA Polymerase theta protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:20000
• Reference: Sharief F.S., Genomics 59:90-96(1999).
  Harris P.V., Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.

Target Info

• Gene Symbol: POLQ
• Gene Full Name: DNA polymerase theta
• Alias Symbols: DNA polymerase eta;DNA polymerase theta;epididymis secretory sperm binding protein;polymerase (DNA directed), theta;polymerase (DNA) theta;PRO0327.
• NCBI Gene Id: 10721
• Protein Name: DNA polymerase theta
• Description of Target: DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity).
• Uniprot ID: O75417
• Protein Accession #: NP_955452.3
• Molecular Weight: 197 kDa