PLK2 Antibody - C-terminal region (OAAB17037)

Catalog No: OAAB17037

Size:400ul

The anti-SNK C-term Pab (Cat. # OAAB17037) is used in Western blot to detect SNK in PMA-treated Pam212 cell lysate (lane 1) and rat testis tissue lysate (lane 2).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SNK Antibody (C-term ) (Cat.# OAAB17037), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: PLK2 (SNK) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress
  Cellular Location: Cytoplasm, cytoskeleton, centrosome, centriole. Cell projection, dendrite (By similarity) Note=Localizes to centrosomes during early G1 phase where it only associates to the mother centriole and then distributes equally to both mother and daughter centrioles at the onset of S phase
  Tissue Location: Expressed at higher level in the fetal lung, kidney, spleen and heart
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PLK2 (SNK) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375-406 amino acids from the C-terminal region of human PLK2 (SNK).
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: PLK2
• Alias Symbols: SNK, hSNK, hPlk2
• NCBI Gene Id: 10769
• Protein Name: Serine/threonine-protein kinase PLK2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9NYY3
• Protein Accession #: NP_001239155.1
• Molecular Weight: 78237 Da