PLEKHM1 Antibody (OAPB00993)

Catalog No: OAPB00993

Size:100UG

Western blot analysis of PLEKHM1 in human lung tissue lysate with PLEKHM1 antibody at 1 μg/mL.

Immunohistochemistry of PLEKHM1 in human lung tissue with PLEKHM1 antibody at 5 μg/mL.

Immunofluorescence of PLKHM1 in human lung tissue with PLKHM1 antibody at 20 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PLEKHM1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: PLEKHM1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: PLEKHM1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human PLEKHM1. The immunogen is located within amino acids 50 - 100 of PLEKHM1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-PLEKHM1 antibody is Catalog # OEPB03120
• Application Info: PLEKHM1 antibody can be used for detection of PLEKHM1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Van Wesenbeeck L, Odgren PR, Mackay CA, et al. Localization of the gene causing the osteopetrotic phenotype in the incisors absent (ia) rat on chromosome 10q32.1. J. Bone Miner. Res.2004; 19:183-9.;Van Wesenbeeck L, Odgren PR, Coxon FP, et al. Involvement of PLEKHM1 in osteoclastic vesicular transport and osteopetrosis in incisors absent rats and humans. J. Clin. Invest.2007; 117:919-30.;Del Fattore A, Fornari R, Van Wesenbeeck L, et al. A new heterozygous mutation (R714C) of the osteopetrosis gene, pleckstrin homolog domain containing family M (with run domain) member 1 (PLEKHM1), impairs vesicular acidification and increases TRACP secretion in osteoclasts. J. Bone Miner. Res.2008; 23:380-91.;

Target Info

• Gene Symbol: PLEKHM1
• Gene Full Name: pleckstrin homology and RUN domain containing M1
• Alias Symbols: 162 kDa adapter protein;AP162;B2;OPTA3;OPTB6;PH domain-containing family M member 1;pleckstrin homology domain containing, family M (with RUN domain) member 1;pleckstrin homology domain-containing family M member 1.
• NCBI Gene Id: 9842
• Protein Name: Pleckstrin homology domain-containing family M member 1
• Description of Target: Proposed to act as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Seems to be involved in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (PubMed:25498145). Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity). May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7 (PubMed:20943950). May have a role in sialyl-lex-mediated transduction of apoptotic signals (PubMed:12820725). Involved in bone resorption (By similarity). In case of infection contributes to Salmonella typhimurium pathogenesis by supporting the integrity of the Salmonella-containing vacuole (SCV) probably in concert with the HOPS complex and Rab7 (PubMed:25500191).
• Uniprot ID: Q9Y4G2