PIP5K1C Antibody (OAAF04008)

Catalog No: OAAF04008 (Formerly GWB-ASE834)

Size:100 ug

Western blot analysis of extracts from A549 cells, using PIP5K1C Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human PIP5K1C.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: PIP5K1C antibody detects endogenous levels of total PIP5K1C protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; ELISA: 1:20000
• Reference: Nagase T., DNA Res. 5:31-39(1998).
  Grimwood J., Nature 428:529-535(2004).
  Di Paolo G., Nature 420-85-89(2002).

Target Info

• Gene Symbol: PIP5K1C
• Gene Full Name: phosphatidylinositol-4-phosphate 5-kinase type 1 gamma
• Alias Symbols: diphosphoinositide kinase;LCCS3;Phosphatidylinositol 4-phosphate 5-kinase type I gamma;phosphatidylinositol 4-phosphate 5-kinase type-1 gamma;phosphatidylinositol-4-phosphate 5-kinase, type I, gamma;PIP5K1gamma;PIP5K1-gamma;PIP5Kgamma;PIP5K-GAMMA;ptdIns(4)P-5-kinase 1 gamma;type I phosphatidylinositol 4-phosphate 5-kinase gamma;type I PIP kinase.
• NCBI Gene Id: 23396
• Protein Name: Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
• Description of Target: Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth.
• Uniprot ID: O60331
• Protein Accession #: NP_001182662.1
• Molecular Weight: 73 kDa