PIIINP ELISA Kit (Human) (OKEH00548)

Catalog No: OKEH00548

Size:96T

Human PIIINP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PIIINP ELISA Kit (Human) (OKEH00548) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog PIIINP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PIIINP and incubated. The PIIINP found in the sample or standards competes with the biotinylated PIIINP for limited binding sites on the immobilized anti-dog PIIINP antibody. Excess unbound biotinylated PIIINP and sample or standard PIIINP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PIIINP captured in the well and inversely proportional to the amount of PIIINP which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.1% (n = 20)
  Mean Inter-assay CV%: < 8.0% (n = 20)
• ELISA Kit Component:
  • Anti-PIIINP Microplate: 96 Wells (12 x 8 Well strips)
  • PIIINP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X PIIINP-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.21 ng/mL
• Specificity: Natural and recombinant Human N-terminal propeptide of Collagen alpha-1(III) chain
• Assay Info: Assay Methodology: Quantitative Competitive ELISA
• Description:
  View product citations for PIIINP ELISA Kit (Human) (OKEH00548) on CiteAb

Target Info

• Gene Symbol: PIIINP
• Alias Symbols: N-terminal propeptide of Collagen alpha-1(III) chain
• Protein Name: AAH28178.1
• Description of Target: COL3A1 protein [Homo sapiens]
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/AAH28178.1