PFKL ELISA Kit (Human) (OKEH05741)

Catalog No: OKEH05741

Size:96T

Human PFKL ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology PFKL ELISA Kit (Human) (OKEH05741) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PFKL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PFKL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PFKL captured in well.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 95%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <6.9%, n = 20
  Mean Inter-Assay Precision - <9.7%, n = 20
• ELISA Kit Component:
  • PFKL Microplate: 96 Wells (12 x 8 Well strips)
  • PFKL Lyophilized Standard: 2
  • 100X Biotinylated PFKL Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.787 ng/mL
• Specificity: Natural and recombinant Human 6-phosphofructokinase, liver type
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PFKL
• Gene Full Name: phosphofructokinase, liver type
• Alias Symbols: 6-phosphofructokinase type B;6-phosphofructokinase, liver type;ATP-dependent 6-phosphofructokinase, liver type;ATP-PFK;liver-type 1-phosphofructokinase;PFK-B;PFK-L;phosphofructo-1-kinase isozyme B;phosphohexokinase.
• NCBI Gene Id: 5211
• Protein Name: P17858
• Description of Target: ATP-dependent 6-phosphofructokinase, liver type
• Uniprot ID: https://www.uniprot.org/uniprot/P17858
• Protein Accession #: NP_001002021.2
• Nucleotide Accession #: NM_001002021.2
• Protein Size (# AA): 780