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Permeabilization Buffer for Immunocytochemistry and Immunohistochemistry (OOMB00004)

Catalog#: OOMB00004
Domestic: within 1 week delivery | International: 1 week
Permeabilization buffer is a ready-to-use reagent for the permeabilization of cells and tissue sections fixed with chemical fixatives including but not limited to formaline, paraformaldehyde, alcohols and acetone. This buffer is added to fixed cells and tissue sections prior to adding primary antibodies. Incubation is done at room temperature for 15-30 minutes. After that the permeabilization buffer is discarded and primary antibodies can be added without rinsing cells and tissue sections from the buffer. The buffer significantly increases the penetration of primary and secondary antibodies into the cells and facilitate intracellular staining of cytoplasmic and nuclear targets. In addition, this buffer reduces non-specific binding of antibodies to irrelevant targets thus providing stronger detection of low abundant targets by boosting signal-to-noise ratio. This buffer is compatible with chromogenic and fluorescence detection protocols.
More Information
Product Format Liquid. Ready-to-use
Application ICC, IHC, FC
Reconstitution and Storage For long-term storage (1-6 months) permeabilization buffer can stored at -20°C and for short-term storage (1-3 weeks) at 4°C. Do not keep buffer at room temperature for more than 3 days.
Datasheets/Manuals Printable datasheet for OOMB00004
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