PDK2 Antibody - C-terminal region (OABB01146)

Catalog No: OABB01146

Size:100UG

Anti-PDK2 antibody, OABB01146, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: MCF-7 Cell Lysate

Anti-PDK2 antibody, OABB01146, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti-PDK2 antibody, OABB01146, IHC(P)
IHC(P): Rat Brain Tissue

Anti-PDK2 antibody, OABB01146, ICC
ICC: HELA Cell

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunocytochemistry|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: PDK2 (Pyruvate Dehydrogenase Kinase Isoenzyme 2), is an enzyme that in humans is encoded by the PDK2 gene. This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
  Immunocytochemistry : 0.5-1 ug/ml: Human: Mouse, Rat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial(PDK2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Target Info

• Gene Symbol: PDK2
• Gene Full Name: pyruvate dehydrogenase kinase 2
• Alias Symbols: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial;PDH kinase 2;PDHK2;PDKII;Pyruvate dehydrogenase kinase isoform 2;pyruvate dehydrogenase kinase, isoenzyme 2;pyruvate dehydrogenase kinase, isozyme 2;pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial.
• NCBI Gene Id: 5164
• Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
• Description of Target: Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.
• Uniprot ID: Q15119
• Molecular Weight: 46154 MW