Catalog No: OKCD08892
Size:96WELLS
Price: $800.00
SKU
OKCD08892
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for P2RY12 ELISA Kit (Human) (OKCD08892) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.054ng/mL |
Gene Symbol | P2RY12 |
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Gene Full Name | purinergic receptor P2Y12 |
Alias Symbols | ADP-glucose receptor;ADPG-R;BDPLT8;Gi-coupled ADP receptor HORK3;G-protein coupled receptor SP1999;HORK3;P2T(AC);P2Y purinoceptor 12;P2Y(12)R;P2Y(AC);P2Y(ADP);P2Y(cyc);P2Y12;P2Y12 platelet ADP receptor;purinergic receptor P2RY12;purinergic receptor P2Y, G-protein coupled, 12;putative G-protein coupled receptor;SP1999. |
NCBI Gene Id | 64805 |
Protein Name | P2Y purinoceptor 12 |
Description of Target | Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation. |
Uniprot ID | Q9H244 |
Protein Accession # | NP_073625.1 |
Nucleotide Accession # | NM_022788.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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