P2RX7 Chemi-Luminescent ELISA Kit (Human) (OKCD03848)

Catalog No: OKCD03848

Size:96WELLS

Human P2RX7 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • P2RX7 Microplate: 96 Wells (12 x 8 Well strips)
  • P2RX7 Lyophilized Standard: 2
  • 100X P2RX7 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.312 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: P2RX7
• Gene Full Name: purinergic receptor P2X 7
• Alias Symbols: ATP receptor;P2X purinoceptor 7;P2X7;P2X7 receptor;P2Z receptor;Purinergic receptor;purinergic receptor P2X, ligand gated ion channel, 7;purinergic receptor P2X7 variant A;purnergic receptor P2X 7.
• NCBI Gene Id: 5027
• Protein Name: P2X purinoceptor 7
• Description of Target: Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells (PubMed:21821797, PubMed:23303206).
• Uniprot ID: Q99572
• Protein Accession #: NP_002553
• Nucleotide Accession #: NM_002562