NUCB2 ELISA Kit (Human) (OKCD06173)

Catalog No: OKCD06173

Size:96WELLS

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nesfatin 1 (NES1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nesfatin 1 (NES1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nesfatin 1 (NES1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nesfatin 1 (NES1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nesfatin 1 (NES1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nesfatin 1 (NES1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • NUCB2 Microplate: 96 Wells (12 x 8 Well strips)
  • NUCB2 Lyophilized Standard: 2
  • 100X NUCB2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C

Target Info

• Gene Symbol: NUCB2
• Gene Full Name: nucleobindin 2
• Alias Symbols: DNA-binding protein NEFA;epididymis secretory protein Li 109;gastric cancer antigen Zg4;HEL-S-109;NEFA;nesfatin 1;novel DNA binding/EF-hand/leucine zipper protein;nucleobindin-2;nucleobinding 2;prepronesfatin.
• NCBI Gene Id: 4925
• Protein Name: Nucleobindin-2
• Description of Target: Calcium-binding protein. May have a role in calcium homeostasis.
• Uniprot ID: P80303
• Protein Accession #: NP_005004.1
• Nucleotide Accession #: NM_005013.2