Size:96 Wells
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NRTN ELISA Kit (Mouse) (OKEH03574)

Catalog#: OKEH03574
Domestic: within 1 week delivery ,International: 1 week
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology NRTN ELISA Kit (Mouse) (OKEH03574) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nrtn has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Nrtn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nrtn captured in well.
Kit Range 78 - 5000pg/ml
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Kit Reproducibility Mean Intra-assay CV%: <= 6.7% (n = 20)
Mean Inter-assay CV%: <= 8.5% (n = 20)
Kit Component
Nrtn Microplate96 Wells (12 x 8 Well strips)
Nrtn Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Nrtn Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 40.2 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse Neurturin
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol NRTN
Official Gene Full Name neurturin
Alias Symbols Neurturin
NCBI Gene Id 18188
Protein Name Neurturin
Description of Target Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
Swissprot Id P97463
Protein Accession # NP_032764.1
Nucleotide Accession # NM_008738.2
Protein Size (# AA) 195
Molecular Weight 22 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Nrtn.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Nrtn.
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