Size:96 Wells
$469.00
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SKU
OKEH02991
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NPY ELISA Kit (Mouse) (OKEH02991)

Catalog#: OKEH02991
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology NPY ELISA Kit (Mouse) (OKEH02991) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Npy antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Npy and incubated. The Npy found in the sample or standards competes with the biotinylated Npy for limited binding sites on the immobilized anti-mouse Npy antibody. Excess unbound biotinylated Npy and sample or standard Npy is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Npy captured in the well and inversely proportional to the amount of Npy which was contained in the sample or standard.
Kit Range 15.6 - 1000pg/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Kit Reproducibility Mean Intra-assay CV%: <7.4% (n = 20)
Mean Inter-assay CV%: <9.5% (n = 20)
Kit Component
ComponentAmount
Anti-Npy Microplate96 Wells (12 x 8 Well strips)
Npy Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Npy-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity 7.98 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse Pro-neuropeptide Y
Assay Info Assay Methodology: Quantitative Competitive ELISA
Gene Symbol NPY
Official Gene Full Name neuropeptide Y
Alias Symbols 0710005A05Rik, Neuropeptide Y
NCBI Gene Id 109648
Protein Name Pro-neuropeptide Y
Description of Target NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Swissprot Id P57774
Protein Accession # NP_075945.1
Nucleotide Accession # NM_023456.3
Protein Size (# AA) 97
Molecular Weight 11 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Npy.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Npy.
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