NOV ELISA Kit (Human) (OKBB00827)

Catalog No: OKBB00827

Size:96 wells

NOV ELISA Kit (Human) (OKBB00827) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human CCN3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CCN3 has been precoated onto 96-well plates. Standards(NS0, T32-M357) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCN3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCN3 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	66	265	820	61	258	754
  Standard deviation	2.77	16.43	37.72	3.41	19.86	45.24
  CV(%)	4.2	6.2	4.6	5.6	7.7	6

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human NOV Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human NOV Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human NOV Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	66	61	66	62	63	2.27	3.6%
  Sample 2	265	294	297	292	287	12.82	4.4%
  Sample 3	820	835	750	802	801	32.08	4%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
  O.D.	0.055	0.094	0.110	0.172	0.327	0.560	1.055	1.926

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <2 pg/ml
• Specificity: Natural and recombinant human CCN3
• Immunogen: Expression system for standard: NS0; Immunogen sequence: T32-M357
• Description: For quantitative detection of human CCN3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CCN3
• Gene Full Name: cellular communication network factor 3
• Alias Symbols: ccn 3, CCN family member 3, CCN3, IGFBP9, NOVH, IBP-9, IGF-binding protein 9, IGFBP9, IGFBP-9, nephroblastoma overexpressed, NovH, P48745, Protein NOV homolog
• NCBI Gene Id: 4856
• Protein Name: CCN family member 3
• Description of Target: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.
• Uniprot ID: P48745
• Protein Accession #: NP_002505.1
• Nucleotide Accession #: NM_002514.3