NOTCH1 Antibody - C-terminal region (OAAB21539)

Catalog No: OAAB21539

Size:400ul

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Clone: RB2197/2198
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: IHC-P
• Additional Information: Function: NOTCH1 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. This protein effects the implementation of differentiation, proliferation and apoptotic programs, and may be important for normal lymphocyte function. In altered form, it may contribute to transformation or progression in some T-cell neoplasms. In fetal tissues it is most abundant in spleen, brain stem and lung. NOTCH1 is also present in most adult tissues where it is found mainly in lymphoid tissues. NOTCH1 is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. NOTCH1 contains a putative 5 ANK repeats and 36 EGF-like domains.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This NOTCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human NOTCH1.
• Purification: SAS precipitation, followed by dialysis against PBS
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Concentration: 2 mg/mL
• Preservative: 0.09% Sodium Azide
• Formulation: Purified polyclonal antibody, supplied in PBS with 0.09% sodium azide.
• Application Info: IHC-P: 1:50~100
• Reference: Gray, G.E., et al., Am. J. Pathol. 154(3):785-794 (1999). Matsuno, K., et al., Nat. Genet. 19(1):74-78 (1998). Ellisen, L.W., et al., Cell 66(4):649-661 (1991).

Target Info

• Gene Symbol: NOTCH1
• Gene Full Name: notch 1
• Alias Symbols: hN1, AOS5, TAN1, AOVD1
• NCBI Gene Id: 4851
• Description of Target: This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
• Uniprot ID: P46531
• Protein Accession #: NP_060087.3
• Nucleotide Accession #: NM_017617.3
• Molecular Weight: 273 kDa