NFE2L1 ELISA Kit (Chicken) (OKEH08108)

Catalog No: OKEH08108

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology NFE2L1 ELISA Kit (Chicken) (OKEH08108) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NFE2L1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NFE2L1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NFE2L1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.4% (n = 20)
  Mean Inter-assay CV%: <=12.1% (n = 20)
• ELISA Kit Component:
  • NFE2L1 Microplate: 96 Wells (12 x 8 Well strips)
  • NFE2L1 Lyophilized Standard: 2
  • 100X Biotinylated NFE2L1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Transcription factor NRF1: CNC-type bZIP family transcription factor that translocates to the nucleus and regulates expression of target genes in response to various stresses. Heterodimerizes with small-Maf proteins (MAFF, MAFG or MAFK) and binds DNA motifs including the antioxidant response elements (AREs), which regulate expression of genes involved in oxidative stress response. Activates or represses expression of target genes, depending on the context (By similarity). Plays a key role in cholesterol homeostasis by acting as a sensor of cholesterol excess: in low cholesterol conditions, translocates into the nucleus and represses expression of genes involved in defense against cholesterol excess (By similarity). In excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal (By similarity). Critical for redox balance in response to oxidative stress: acts by binding the AREs motifs on promoters and mediating activation of oxidative stress response genes (By similarity). Involved in proteasome homeostasis: in response to proteasome inhibition, mediates the 'bounce-back' of proteasome subunits by translocating into the nucleus and activating expression of genes encoding proteasome subunits (By similarity).
  Subcellular Location: Transcription factor NRF1 Nucleus Translocates into the nucleus following cleavage of Endoplasmic reticulum membrane sensor NFE2L1.
  Unigene: Gga.31932
  SMR: Q5ZL67
  String: 9031.ENSGALP00000019444
  KEGG: gga:417987
  Pfam: PF03131
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Endoplasmic reticulum membrane sensor NFE2L1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NFE2L1
• Gene Full Name: nuclear factor, erythroid 2 like 1
• Alias Symbols: endoplasmic reticulum membrane sensor NFE2L1;NFE2-related factor 1;NF-E2-related factor 1;nuclear factor (erythroid-derived 2)-like 1;nuclear factor erythroid 2-related factor 1;Nuclear factor, erythroid derived 2, like 1.
• NCBI Gene Id: 417987
• Protein Name: Q5ZL67
• Description of Target: Endoplasmic reticulum membrane sensor NFE2L1
• Uniprot ID: https://www.uniprot.org/uniprot/Q5ZL67
• Protein Accession #: NP_001025927.1
• Nucleotide Accession #: NM_001030756.1