Catalog No: OKRC01777
Size:96 Wells
Price: $537.00
SKU
OKRC01777
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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NF-KappaB p65 Transcription Factor Activity Assay (Human) (OKRC01777)
Datasheets/Manuals | Printable datasheet for OKRC01777 |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Application | ELISA |
ELISA Kit Detection Method | Colorimetric, OD450 nm |
ELISA Kit Component |
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Additional Information | Pathway: B Cell Receptor, Inflammation, NFkB Signaling, T Cell Receptor |
:: | Other materials required:
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Reconstitution and Storage | Upon receipt, the positive control should be removed and stored at -20 or -80C. The remainder of the kit can be stored for up to 6 months at 2-8C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2 to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20C upon receipt. Avoid repeated freeze-thaw cycles. |
Sample Type | Cell Lysates, Nuclear Extracts |
Specificity | The olionucleotide/antibody pair provided in this kit recognizes human NF-?B p65 in whole lysates and nuclear extracts. |
Assay Info | Assay Methodology: Semi-Quantitative Sandwich ELISA |
Introduction | NF-kB is a transcription factor that plays a pivotal role in a number of physiological cell processes such as growth, apoptosis, and immune response through regulating the expression of a number of cellular genes. The NF-kB family includes five members: cRel, RelB, p65(RelA), p50(p105, precursor of p50), and p52(p100, precursor of p52), which form homo- or heterodimers when in active status. Normally, NF-kB is inactive and retained in the cytoplasm bound to its inhibitory protein IkB. Upon stimulation of various factors such as infection of pathogens including viruses and bacteria or treatment of cytokines such as IL-1 or TNF-a, IkB proteins are phosphorylated, ubiquitinated and finally degraded. Released NF-kB proteins then form homo- or heterodimers, most commonly p50/p65 or p50/p50, which are transported into the nucleus where they bind to specific DNA sequences of targeted genes and activate transcription. NF-kB also induces expression of its own inhibitor, IkBa, which binds to active NF-kB in the nucleus to terminate the induction of gene expression. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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