Neopterin ELISA kit (Human) (OKWB10530)

Catalog No: OKWB10530

Size:96T

Human Neopterin ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Neopterin ELISA Kit (Human) (OKEH00637) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Neopterin antibody and blocked. Sample or standards are added to the wells along with a fixed quantity of biotinylated Neopterin and incubated. The Neopterin found in the sample or standards competes with the biotinylated Neopterin for limited binding sites on the immobilized anti-Neopterin antibody. Excess unbound biotinylated Neopterin and sample or standard Neopterin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Neopterin captured in the well and inversely proportional to the amount of Neopterin which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Neopterin was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Serping1 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Neopterin Microplate: 96 Wells (12 x 8 Well Strips)
  • Neopterin Lyophilized Standard: 2 x 20 ng
  • 100X Neopterin Biotin Complex: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Additional Information: CAS: 2009-64-5
  PubChem: 448839
  Molecular Formula: C9H11N5O4
  
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.094 ng/mL
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Alias Symbols: 2-amino-6-(1,2,3-trihydroxypropyl)-1H-pteridin-4-one,D-(+)-Neopterin, D-Neopterin, Neopterin, D-erythro-Neopterin, 2009-64-5, 6-D-erythro-Neopterin
• Protein Name: Neopterin
• Description of Target: Neopterin is a catabolic product of guanosine triphosphate (GTP), a purine nucleotide. Neopterin belongs to the chemical group known as pteridines. It is synthesised by human macrophages upon stimulation with the cytokine interferon-gamma and is indicative of a pro-inflammatory immune status. Neopterin serves as a marker of cellular immune system activation.
• Protein Size (# AA): 253.215 g/mol