MYH10 ELISA Kit (Human) (OKCD08481)

Catalog No: OKCD08481

Size:96WELLS

MYH10 ELISA Kit (Human) (OKCD08481) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 10, Non Muscle (MYH10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 10, Non Muscle (MYH10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 10, Non Muscle (MYH10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myosin Heavy Chain 10, Non Muscle (MYH10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 10, Non Muscle (MYH10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 10, Non Muscle (MYH10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MYH10 Microplate: 96 Wells (12 x 8 Well strips)
  • MYH10 Lyophilized Standard: 2
  • 100X MYH10 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.055ng/mL

Target Info

• Gene Symbol: MYH10
• Gene Full Name: myosin heavy chain 10
• Alias Symbols: Cellular myosin heavy chain, type B;Myosin heavy chain 10;Myosin heavy chain, non-muscle IIb;myosin heavy chain, nonmuscle type B;myosin, heavy chain 10, non-muscle;myosin, heavy polypeptide 10, non-muscle;myosin-10;NMMHCB;NMMHC-IIB;Non-muscle myosin heavy chain B;nonmuscle myosin heavy chain IIB;Non-muscle myosin heavy chain IIb;nonmuscle myosin II heavy chain-B.
• NCBI Gene Id: 4628
• Protein Name: Myosin-10
• Description of Target: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9.
• Uniprot ID: P35580
• Protein Accession #: NP_001242941.1
• Nucleotide Accession #: NM_001256012.1