MT3 ELISA Kit (Human) (OKDD00405)

Catalog No: OKDD00405 (Formerly GWB-KBBAN6)

Size:96 Wells

Human MT3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology MT3 ELISA Kit (Human) (OKDD00405) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MT3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MT3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MT3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MT3 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MT3 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-MT3 Microplate: 96 Wells (12 x 8 Well Strips)
  • MT3 Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X MT3 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of MT3. No significant cross-reactivity or interference between MT3 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MT3
• Gene Full Name: metallothionein 3
• Alias Symbols: GIF;GIFB;GRIF;growth inhibitory factor;metallothionein 3 (growth inhibitory factor (neurotrophic));metallothionein-3;metallothionein-III;ZnMT3.
• NCBI Gene Id: 4504
• Protein Name: Metallothionein-3
• Description of Target: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.
• Uniprot ID: P25713
• Protein Accession #: NP_005945.1
• Nucleotide Accession #: NM_005954.2
• Protein Size (# AA): 68
• Molecular Weight: 7 kDa