Catalog No: OKCD09074
Size:96WELLS
Price: $800.00
SKU
OKCD09074
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for MOGAT2 ELISA Kit (Human) (OKCD09074) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monoacylglycerol-O-acyltransferase 2 (MOGAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monoacylglycerol-O-acyltransferase 2 (MOGAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monoacylglycerol-O-acyltransferase 2 (MOGAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Monoacylglycerol-O-acyltransferase 2 (MOGAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monoacylglycerol-O-acyltransferase 2 (MOGAT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monoacylglycerol-O-acyltransferase 2 (MOGAT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.056ng/mL |
Gene Symbol | MOGAT2 |
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Gene Full Name | monoacylglycerol O-acyltransferase 2 |
Alias Symbols | 2-acylglycerol O-acyltransferase 2;acyl-CoA:monoacylglycerol acyltransferase 2;DGAT2L5;DGAT2L5.;diacylglycerol acyltransferase 2-like protein 5;diacylglycerol O-acyltransferase candidate 5;hDC5;MGAT2;Monoacylglycerol O-acyltransferase 2. |
NCBI Gene Id | 80168 |
Protein Name | 2-acylglycerol O-acyltransferase 2 |
Description of Target | Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity. |
Uniprot ID | Q3SYC2 |
Protein Accession # | NP_079374.2 |
Nucleotide Accession # | NM_025098.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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