MMP7 Chemi-Luminescent ELISA Kit (Horse) (OKCD03792)

Catalog No: OKCD03792

Size:96WELLS

Horse MMP7 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Equus caballus|Horse
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	91-101%	81-92%	86-93%
  Heparin Plasma (n=5)	81-96%	85-95%	98-105%	98-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 7 (MMP7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 7 (MMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 7 (MMP7) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-105	101
  Heparin Plasma (n=5)	90-102	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Mmp7 Microplate: 96 Wells (12 x 8 Well strips)
  • Mmp7 Lyophilized Standard: 2
  • 100X Mmp7 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.312 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP7
• Gene Full Name: matrix metallopeptidase 7
• Alias Symbols: matrilysin;matrix metallopeptidase 7 (matrilysin, uterine);matrix metalloproteinase 7.
• NCBI Gene Id: 100068985
• Protein Name: Submitted name: Matrix metalloproteinase 7
• Uniprot ID: Q4JIK0