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MMP17 ELISA Kit (Human) : 96 Wells (OKEH00523)

Catalog#: OKEH00523
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology MMP17 ELISA Kit (Human) (OKEH00523) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human MMP17 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human MMP17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human MMP17 captured in well.
Kit Range 0.312 - 20ng/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Kit Reproducibility Mean Intra-assay CV%: < 3.5% (n = 20)
Mean Inter-assay CV%: < 7.0% (n = 20)
Kit Component
Anti-MMP17 Microplate96 Wells (12 x 8 Well strips)
MMP17 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated MMP171 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 0.21 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Human Matrix metalloproteinase-17
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol MMP17
Official Gene Full Name matrix metallopeptidase 17
Alias Symbols Matrix metalloproteinase-17, Membrane-type-4 matrix metalloproteinase, Membrane-type matrix metalloproteinase 4, MMP-17, MT4MMP, MT4-MMP, MTMMP4, MT-MMP 4
NCBI Gene Id 4326
Protein Name Matrix metalloproteinase-17
Description of Target Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
Swissprot Id Q9ULZ9
Protein Accession # NP_057239.4
Nucleotide Accession # NM_016155.4
Protein Size (# AA) 603
Molecular Weight 67 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express MMP17.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express MMP17.
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