MIA2 ELISA Kit (Human) (OKEH07067)

Catalog No: OKEH07067

Size:96T

Human MIA2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology MIA2 ELISA Kit (Human) (OKEH07067) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-MIA2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MIA2 and incubated. The MIA2 found in the sample or standards competes with the biotinylated MIA2 for limited binding sites on the immobilized anti-MIA2 antibody. Excess unbound biotinylated MIA2 and sample or standard MIA2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MIA2 captured in the well and inversely proportional to the amount of MIA2 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 94.9%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <7.3%
  Inter-Assay CV <10.7%
• ELISA Kit Component:
  • MIA2 Microplate: 96 Wells (12 x 8 Well strips)
  • MIA2 Lyophilized Standard: 2 x 20 ng
  • 100X MIA2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.176 ng/mL
• Specificity: Natural and recombinant Human Melanoma inhibitory activity protein 2
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: MIA2
• Gene Full Name: MIA SH3 domain ER export factor 2
• Alias Symbols: CTAGE family member 5, ER export factor;CTAGE family, member 5;CTAGE5;cutaneous T-cell lymphoma-associated antigen 5;MEA6;melanoma inhibitory activity 2;melanoma inhibitory activity protein 2;meningioma expressed antigen 6 (coiled-coil proline-rich);meningioma-expressed antigen 6/11;MGEA;MGEA11;MGEA6;MIA protein 2;protein cTAGE-5;TALI;TANGO1-like.
• NCBI Gene Id: 4253
• Protein Name: Q96PC5
• Description of Target: Melanoma inhibitory activity protein 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q96PC5
• Protein Accession #: NP_001316143
• Nucleotide Accession #: NM_001329214