MGMT ELISA Kit (Bovine) (OKEH08468)

Catalog No: OKEH08468

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology MGMT ELISA Kit (Bovine) (OKEH08468) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MGMT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MGMT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MGMT captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.0% (n = 20)
  Mean Inter-assay CV%: <=11.1% (n = 20)
• ELISA Kit Component:
  • MGMT Microplate: 96 Wells (12 x 8 Well strips)
  • MGMT Lyophilized Standard: 2
  • 100X Biotinylated MGMT Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
  Subcellular Location: Nucleus
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Methylated-DNA--protein-cysteine methyltransferase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MGMT
• Alias Symbols: 6-O-methylguanine-DNA methyltransferase;O-6-methylguanine-DNA-alkyltransferase.
• Protein Name: P29177
• Description of Target: Methylated-DNA--protein-cysteine methyltransferase
• Uniprot ID: https://www.uniprot.org/uniprot/P29177