Metallothionein ELISA Kit (Human) : 96 Wells (OKCA00004)

Catalog No: OKCA00004

Size:96 Wells

Human Metallothionein ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Human Serum (n=4)	1:50	90%	82-101%
  Human Serum (n=4)	1:100	94%	90-99%
  Human Serum (n=4)	1:200	93%	85-97%
  Human Serum (n=4)	1:400	98%	92-112%

  Sample Type 2
  Sample Type	Dilution	Average %	Range %
  Human Urine (n=4)	1:1	97%	90-108%
  Human Urine (n=4)	1:2	93%	88-97%
  Human Urine (n=4)	1:4	95%	89-104%
  Human Urine (n=4)	1:8	92%	88-104%

  Sample Type 3
  Sample Type	Dilution	Average %	Range %
  Human Cell Culture (n=4)	1:1	95%	81-108%
  Human Cell culture (n=4)	1:2	92%	87-101%
  Human Cell culture (n=4)	1:4	97%	85-114%
  Human Cell culture (n=4)	1:8	95%	90-100%

  Sample Type 3
  Sample Type	Dilution	Average %	Range %
  Human Saliva (n=4)	1:1	94%	86-107%
  Human Saliva (n=4)	1:2	99%	91-115%
  Human Saliva (n=4)	1:4	91%	85-95%
  Human Saliva (n=4)	1:8	92%	87-99%

• ELISA Kit Principle: Aviva Systems Biology Metallothionein ELISA Kit (Human) (OKCA00004) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human Metallothionein has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human Metallothionein is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human Metallothionein captured in well.
• ELISA Kit Range: 6.25 pg/ml - 400 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (ng/ml)	50.469	52.058	50.8	49.567	51.384	48.838
  SD	0.036	0.028	0.027	0.08	0.088	0.057
  CV%	3.367	2.574	2517	7.561	8.146	5.436

• ELISA Kit Component:
  • Anti-MT Microplate: 96 Well (12 x 8 Well Strips)
  • MT Standard (Lyophilized): 2 x 0.4 ng
  • 100X Biotinylated Anti-MT Detector Antibody: 1 x 120 uL
  • 100X HRP-Avidin: 1 x 120 uL
  • Detection Antibody Diluent: 1 x 15 mL
  • HRP-Avidin Diluent: 1 x 15 mL
  • Sample Diluent: 1 x 50 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernates, Urine, Saliva,
  Tissue Homogenates
• Sensitivity: < 0.813 pg/ml
• Specificity: This assay has high sensitivity and excellent specificity for detection of human
  MT. No significant cross-reactivity or interference between human MT and
  analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Metallothionein
• Gene Full Name: Metallothionein 1A
• Alias Symbols: MT1, MTC, MT1S, MT-1A, MT-IA
• NCBI Gene Id: 4489
• Protein Name: Metallothionein-1A
• Uniprot ID: P04731, Q86YX5
• Protein Accession #: NP_005937
• Nucleotide Accession #: NM_005946
• Protein Size (# AA): 61
• Molecular Weight: 6 kDa